You can rely on the Ziplex System for reproducible, reliable and accurate results for gene-expression analysis. The following performance data represents typical results you can expect on the
Ziplex System.

Ziplex signal intensities vary over more than a 1000-fold range of analyte concentrations.

The plot shows the Ziplex signal intensity (log SI) vs. the concentration (log pM) of six spiked-in synthetic oligonucleotides with a single biotin label per molecule, each diluted from 0.1 pM to 316 pM (3x103-fold range). The mean value and standard deviation are plotted for the six spikes.

The limit of detection (LoD) of oligonucleotide targets on the Ziplex System is ~ 0.2 pM. The dynamic range of the Ziplex System is three (3) logs of analyte concentration.

The analytical sensitivity or limit of detection (LoD) of synthetic oligonucleotides (with a single biotin label per molecule) on the Ziplex System was estimated by assessing the variance of probe signals from multiple blanks (chips hybridized with no spikes) and the variance of probe signals from chips with spike concentrations near the limit of detection. The limit of blanks (LoB) in the plot indicates the 95th percentile of the measured blank values. The LoD indicated (0.22 pM) is the lowest amount of analyte that will be detected above blanks with 95% confidence. The dynamic range based on this LoD is about three logs.

Expression measurements made on multiple Ziplex Workstations and TipChip lots are consistent even without normalization.

The overall variance of the un-normalized signals generated on multiple Ziplex Workstations and two chip lots was 19.4% across all probes on the TipChip array, with a median %CV of 16.9%. The median %CV was slightly greater for low signal intensities (SI).

Results from technical replicates are very similar on the Ziplex System, enabling the measurement of small expression differences in research studies.

The normalized signal intensities (SI) from two technical replicates of separate aliquots of Universal Human Reference RNA are correlated in the plot. The correlation coefficient (R) is almost 1.0, indicating excellent repeatability.

The normalized signal intensities (SI) from five technical replicates of separate aliquots of both Universal Human Reference RNA ("A") and Human Brain RNA ("B") are correlated for all pairs of the replicates of each sample. The minimum correlation coefficient was 0.983, indicating consistently excellent repeatability.

Differential expression patterns discovered on the Affymetrix platform will also be observed on focused arrays on the Ziplex System.

Comparison of differential expression between mixtures of Universal Human Reference RNA and Human Brain RNA (mass ratios of 75:25, "C" and 25:75, "D") measured with the Ziplex System and the Affymetrix platform. Only small differences of expression are expected between the two mixtures (less than three-fold). Statistically significant differences are indicated by black symbols. Open symbols indicate genes for which significant signals were not present on one or both of the platforms. The dotted square indicates expression differences between the C and D mixtures of less than 50%.

Differential expression patterns discovered on global expression platforms will be observed on focused arrays on the Ziplex System.

Comparison of differential expression between mixtures of Universal Human Reference RNA and Human Brain RNA (mass ratios of 75:25, "C" and 25:75, "D") measured with Ziplex and three global expression platforms: Affymetrix ("AFX"), Illumina ("ILM") and Agilent ("AGI"). Only small differences of expression are expected between the two mixtures (less than three-fold). Statistically significant differences are indicated by black symbols. Open symbols indicate genes for which significant signals were not present on one or both of the platforms in each plot. The dotted square indicates expression differences between the C and D mixtures of less than 50%.