Sex-based dimorphism of anticancer immune response and molecular mechanisms

Embryonal rhabdomyosarcoma of the uterine corpus: a clinicopathological and molecular analysis of 21 cases highlighting a frequent association with DICER1 mutations

Herein we evaluated a series of 21 embryonal rhabdomyosarcomas of the uterine corpus (ucERMS), a rare neoplasm, to characterize their morphology, genomics, and behavior. Patients ranged from 27 to 73 (median 52) years and tumors from 4 to 15 (median 9) cm, with extrauterine disease noted in two.

Follow-up (median 16 months) was available for 14/21 patients; nine were alive and well, four died of disease, and one died from other causes. Most tumors (16/21) showed predominantly classic morphology, comprised of alternating hyper- and hypocellular areas of primitive small cells and differentiating rhabdomyoblasts in a loose myxoid/edematous stroma.

A cambium layer was noted in all; seven had heterologous elements (six with fetal-type cartilage) and eight displayed focal anaplasia. The remaining five neoplasms showed only a minor component (≤20%) of classic morphology, with anaplasia noted in four and tumor cell necrosis in three. The most frequent mutations detected were in DICER1 (14/21), TP53 (7/20), PI3K/AKT/mTOR pathway (7/20), and KRAS/NRAS (5/20). Copy-number alterations were present in 10/19 tumors.

Overall, 8/14 DICER1-associated ucERMS showed concurrent loss of function and hotspot mutations in DICER1, which is a feature more likely to be seen in tumors associated with DICER1 syndrome.

Germline data were available for two patients, both DICER1 wild type (one with concurrent loss of function and hotspot alterations). DICER1-associated ucERMS were more likely to show a classic histological appearance including heterologous elements than DICER1-independent tumors.

No differences in survival were noted between the two groups, but both patients with extrauterine disease at diagnosis and two with recurrences died from disease. As no patients had a known personal or family history of DICER1 syndrome, we favor most DICER1-associated ucERMS to be sporadic.

 

Using molecular dynamics simulations to prioritize and understand AI-generated cell penetrating peptides

Cell-penetrating peptides have important therapeutic applications in drug delivery, but the variety of known cell-penetrating peptides is still limited. With a promise to accelerate peptide development, artificial intelligence (AI) techniques including deep generative models are currently in spotlight.

Scientists, however, are often overwhelmed by an excessive number of unannotated sequences generated by AI and find it difficult to obtain insights to prioritize them for experimental validation. To avoid this pitfall, we leverage molecular dynamics (MD) simulations to obtain mechanistic information to prioritize and understand AI-generated peptides.

A mechanistic score of permeability is computed from five steered MD simulations starting from different initial structures predicted by homology modelling. To compensate for variability of predicted structures, the score is computed with sample variance penalization so that a peptide with consistent behaviour is highly evaluated.

Our computational pipeline involving deep learning, homology modelling, MD simulations and synthesizability assessment generated 24 novel peptide sequences. The top-scoring peptide showed a consistent pattern of conformational change in all simulations regardless of initial structures.

As a result of wet-lab-experiments, our peptide showed better permeability and weaker toxicity in comparison to a clinically used peptide, TAT. Our result demonstrates how MD simulations can support de novo peptide design by providing mechanistic information supplementing statistical inference.

xceedmolecular
xceedmolecular

TNF-α Polyclonal Antibody

A71438
  • EUR 302.50
  • EUR 423.50
  • 50 ul
  • 100 ul

Monoclonal STAT6 Antibody (monoclonal) (M01), Clone: 6C10

AMM04145G 0.05mg
EUR 580.8
Description: A Monoclonal antibody against Human STAT6 (monoclonal) (M01). The antibodies are raised in mouse and are from clone 6C10. This antibody is applicable in WB, IHC and IF, E

Monoclonal TXN Antibody (monoclonal) (M04), Clone: 6C10

APR10588G 0.1mg
EUR 580.8
Description: A Monoclonal antibody against Human TXN (monoclonal) (M04). The antibodies are raised in mouse and are from clone 6C10. This antibody is applicable in WB and IF, E

Flotillin-1 Mouse Monoclonal Antibody(6C10)

38095-100ul 100ul
EUR 302.4

Flotillin-1 Mouse Monoclonal Antibody(6C10)

38095-50ul 50ul
EUR 224.4

TNF-α Rabbit pAb

A0277-100ul 100 ul
EUR 369.6

TNF-α Rabbit pAb

A0277-200ul 200 ul
EUR 550.8

TNF-α Rabbit pAb

A0277-20ul 20 ul
EUR 219.6

TNF-α Rabbit pAb

A0277-50ul 50 ul
EUR 267.6

TNF-α Rabbit pAb

A11534-100ul 100 ul
EUR 369.6

TNF-α Rabbit pAb

A11534-200ul 200 ul
EUR 550.8

TNF-α Rabbit pAb

A11534-20ul 20 ul
EUR 219.6

TNF-α Rabbit pAb

A11534-50ul 50 ul
EUR 267.6

TNF-α, His, Human

HY-P7416 50ug
EUR 362.4

TNF-α, His, Mouse

HY-P7417 50ug
EUR 583.2

Mouse TNF-alpha ELISA Kit, 96 tests, Quantitative

100-210-TNF 1 kit
EUR 578.4

Marmoset TNF-α ELISPOT antibody pair

CT962-10 10-plate
EUR 633.6

Marmoset TNF-α ELISPOT antibody pair

CT962-20 20-plate
EUR 1107.6

Monkey TNF-α ELISPOT antibody pair

CT617-10 10-plate
EUR 633.6

Monkey TNF-α ELISPOT antibody pair

CT617-20 20-plate
EUR 1107.6

Human TNF-α ELISPOT antibody pair

CT647-10 10-plate
EUR 633.6

Human TNF-α ELISPOT antibody pair

CT647-20 20-plate
EUR 1107.6

Mouse TNF-α ELISPOT antibody pair

CT661-10 10-plate
EUR 633.6

Mouse TNF-α ELISPOT antibody pair

CT661-20 20-plate
EUR 1107.6

Rat TNF-α ELISA antibody pair

CT704-10 10-plate
EUR 656.4

Rat TNF-α ELISA antibody pair

CT704-20 20-plate
EUR 1118.4

Monkey TNF-α ELISA antibody pair

CT717-10 10-plate
EUR 656.4

Monkey TNF-α ELISA antibody pair

CT717-20 20-plate
EUR 1118.4

Human TNF-α ELISA antibody pair

CT747-10 10-plate
EUR 656.4

Human TNF-α ELISA antibody pair

CT747-20 20-plate
EUR 1118.4

Mouse TNF-α ELISA antibody pair

CT761-10 10-plate
EUR 656.4

Mouse TNF-α ELISA antibody pair

CT761-20 20-plate
EUR 1118.4

Marmoset TNF-α ELISA antibody pair

CT772-10 10-plate
EUR 656.4

Marmoset TNF-α ELISA antibody pair

CT772-20 20-plate
EUR 1118.4

P44/42 MAPK (ERK1/2) Monoclonal Antibody(6C10)

44100-100ul 100ul
EUR 302.4

TNF ? Monoclonal Antibody

EM1197-100ul 100ul
EUR 334.8
Description: A Mouse Monoclonal antibody against TNF ? from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

TNF ? Monoclonal Antibody

EM1197-50ul 50ul
EUR 248.4
Description: A Mouse Monoclonal antibody against TNF ? from Human/ Rat/ Mouse. This antibody is tested and validated for WB, ELISA, IHC

Recombinant Rabbit Tumor Necrosis Factor α/TNF-α

C082-10ug 10ug
EUR 201.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.4.

Recombinant Rabbit Tumor Necrosis Factor α/TNF-α

C082-1mg 1mg
EUR 2739.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.4.

Recombinant Rabbit Tumor Necrosis Factor α/TNF-α

C082-500ug 500ug
EUR 1935.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.4.

Recombinant Rabbit Tumor Necrosis Factor α/TNF-α

C082-50ug 50ug
EUR 486
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 300mM NaCl, pH7.4.

Swine TNF-α ELISA Kit

DEIA-T6108 96T
EUR 639.6
Description: For the quantitative determination of swine tumor necrosis factor(TNF-α) concentrations in cell culture supernates, serum, and plasma.This package insert must be read in its entirety before using this product.

Mouse TNF-α ELISA kit

DEIA7391 96T
EUR 889.2
Description: The Mouse Tumor Necrosis Factor Alpha (TNF-α) ELISA Kit is to be used for the vitro quantitative determination of Mouse TNF-α in serum, plasma, tissue lysates, cell culture supernatants and other biological fluids. The Kit is intended for research use only, not for diagnostic or therapeutic procedure. If detection of other special sample, please contact our technical support.

Marmoset TNF-α ELISPOT kit

CT938-PR2 2-plate
EUR 498

Marmoset TNF-α ELISPOT kit

CT938-PR5 5-plate
EUR 667.2

Rat TNF-α ELISA kit

CT075A 5-plate
EUR 554.4

Monkey TNF-α ELISPOT kit

CT133-PB2 2-plate
EUR 498

Monkey TNF-α ELISPOT kit

CT133-PB5 5-plate
EUR 681.6

Monkey TNF-α ELISPOT kit

CT133-PR2 2-plate
EUR 498

Monkey TNF-α ELISPOT kit

CT133-PR5 5-plate
EUR 667.2

Monkey TNF-α ELISPOT kit

CT133-T2 2-plate
EUR 432

Monkey TNF-α ELISPOT kit

CT133-T5 5-plate
EUR 694.8

Monkey TNF-α ELISA kit

CT148A 5-plate
EUR 554.4

Human TNF-α ELISA kit

CT209A 5-plate
EUR 554.4

Human TNF-α ELISPOT kit

CT237-PB2 2-plate
EUR 498

Human TNF-α ELISPOT kit

CT237-PB5 5-plate
EUR 681.6

Human TNF-α ELISPOT kit

CT237-PR2 2-plate
EUR 498

Human TNF-α ELISPOT kit

CT237-PR5 5-plate
EUR 667.2

Human TNF-α ELISPOT kit

CT237-T2 2-plate
EUR 432

Human TNF-α ELISPOT kit

CT237-T5 5-plate
EUR 694.8

Mouse TNF-α ELISA kit

CT303A 5-plate
EUR 554.4

Mouse TNF-α ELISPOT kit

CT322-PB2 2-plate
EUR 498

Mouse TNF-α ELISPOT kit

CT322-PB5 5-plate
EUR 681.6

Mouse TNF-α ELISPOT kit

CT322-PR2 2-plate
EUR 498

Mouse TNF-α ELISPOT kit

CT322-PR5 5-plate
EUR 667.2

Mouse TNF-α ELISPOT kit

CT322-T2 2-plate
EUR 432

Mouse TNF-α ELISPOT kit

CT322-T5 5-plate
EUR 694.8

Marmoset TNF-α ELISA kit

CT342A 5-plate
EUR 554.4

human TNF-α,His tag

E410A13-10 100μg
EUR 832.8

TNF-α (31-45), human

HY-P1860 1mg
EUR 432

Recombinant Mouse TNF α Protein

RP00816 10 μg
EUR 219.6

α-SMA Monoclonal Antibody

40482-100ul 100ul
EUR 302.4

α-SMA Monoclonal Antibody

40482-50ul 50ul
EUR 224.4

α-SMA Monoclonal Antibody

40495-100ul 100ul
EUR 302.4

α-SMA Monoclonal Antibody

40495-50ul 50ul
EUR 224.4

anti-STAT6 (6C10)

LF-MA10315 50 ug
EUR 435.6
Description: Mouse monoclonal to STAT6

Anti-STAT6 (6C10)

YF-MA15643 200 ul
EUR 435.6
Description: Mouse monoclonal to STAT6

Anti-BRD3 (6C10)

YF-MA16265 50 ug
EUR 435.6
Description: Mouse monoclonal to BRD3

Anti-BRD3 (6C10)

YF-MA16266 200 ul
EUR 435.6
Description: Mouse monoclonal to BRD3

Anti-TNF-α (Golimumab), Human IgG1 Antibody

A2141-100 100 µg
EUR 612

Anti-TNF-α (Certolizumab Pegol), Humanized Antibody

A2142-100 100 µg
EUR 612

Genorise® Sheep TNF-α polyclonal antibody

GR130002 100 µg
EUR 477.6

Genorise® Mouse TNF-α polyclonal antibody

GR131002 100 µg
EUR 477.6

Genorise® Rat TNF-α polyclonal antibody

GR132002 100 µg
EUR 477.6

Genorise® Rabbit TNF-α Polyclonal antibody

GR180018 100 mg
EUR 477.6

Genorise® Monkey TNF-α Polyclonal antibody

GR184062 100 mg
EUR 477.6

Genorise® Hamster TNF-α polyclonal antibody

GR208002 100 µg
EUR 477.6

Genorise® Feline TNF-α polyclonal antibody

GR195002 100 µg
EUR 477.6

Goat Tumor necrosis factor α,TNF-α ELISA KIT

CN-00367S1 96T
EUR 548.4

Goat Tumor necrosis factor α,TNF-α ELISA KIT

CN-00367S2 48T
EUR 367.2

PoFAine tumor necrosis factor α,TNF-α ELISA Kit

CN-01217P1 96T
EUR 564

PoFAine tumor necrosis factor α,TNF-α ELISA Kit

CN-01217P2 48T
EUR 384

Rat Tumor necrosis factor α,TNF-α ELISA KIT

CN-01587R1 96T
EUR 530.4

Rat Tumor necrosis factor α,TNF-α ELISA KIT

CN-01587R2 48T
EUR 351.6

Human Tumor necrosis factor α,TNF-α ELISA KIT

CN-03277H1 96T
EUR 542.4

Human Tumor necrosis factor α,TNF-α ELISA KIT

CN-03277H2 48T
EUR 362.4

Monkey Tumor necrosis factor α,TNF-α ELISA KIT

CN-02231M1 96T
EUR 592.8

Monkey Tumor necrosis factor α,TNF-α ELISA KIT

CN-02231M2 48T
EUR 412.8

Mouse Tumor necrosis factor α,TNF-α ELISA KIT

CN-02415M1 96T
EUR 548.4

Mouse Tumor necrosis factor α,TNF-α ELISA KIT

CN-02415M2 48T
EUR 367.2

Hamster TNF-α(Tumor Necrosis Factor-α) ELISA Kit

EHA0004 96T
EUR 681.12
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Hamster ;Sensitivity: 1.875pg/ml

Rat Tumor necrosis factor α(TNF-α)ELISA Kit

GA-E0772RT-48T 48T
EUR 380.4

Rat Tumor necrosis factor α(TNF-α)ELISA Kit

GA-E0772RT-96T 96T
EUR 595.2

Bovine tumor necrosis factor α(TNF-α)ELISA KIT

GA-E0017BV-48T 48T
EUR 403.2

Bovine tumor necrosis factor α(TNF-α)ELISA KIT

GA-E0017BV-96T 96T
EUR 640.8

Human Tumor necrosis factor α(TNF-α)ELISA Kit

GA-E0122HM-48T 48T
EUR 346.8

Human Tumor necrosis factor α(TNF-α)ELISA Kit

GA-E0122HM-96T 96T
EUR 559.2

Mouse Tumor necrosis factor α(TNF-α)ELISA KIT

GA-E0130MS-48T 48T
EUR 403.2

Mouse Tumor necrosis factor α(TNF-α)ELISA KIT

GA-E0130MS-96T 96T
EUR 640.8

Chicken Tumor necrosis factor α,TNF-α ELISA KIT

QY-E80030 96T
EUR 511.2

Human Tumor necrosis factor α(TNF-α)ELISA Kit

QY-E00182 96T
EUR 433.2

Rat Tumor necrosis factor α(TNF-α)ELISA Kit

QY-E10880 96T
EUR 433.2

Goat Tumor necrosis factor α,TNF-α ELISA KIT

QY-E140007 96T
EUR 495.6

Sheep Tumor necrosis factor α(TNF-α)ELISA Kit

QY-E140060 96T
EUR 495.6

Mouse Tumor necrosis factor α(TNF-α)ELISA Kit

QY-E21543 96T
EUR 433.2

Porcine tumor necrosis factor α,TNF-α ELISA Kit

QY-E40006 96T
EUR 472.8

Bovine Tumor necrosis factor α(TNF-α)ELISA Kit

QY-E60007 96T
EUR 511.2

Canine Tumor necrosis factor α,TNF-α ELISA Kit

QY-E70025 96T
EUR 511.2

Bird Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0002BI-48T 48T
EUR 525

Bird Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0002BI-96T 96T
EUR 645

Duck Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0004DU-48T 48T
EUR 525

Duck Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0004DU-96T 96T
EUR 645

Camel Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0016CM-48T 48T
EUR 525

Camel Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0016CM-96T 96T
EUR 645

Porcine tumor necrosis factor α,TNF-α ELISA Kit

YLA0016PO-48T 48T
EUR 502.5

Porcine tumor necrosis factor α,TNF-α ELISA Kit

YLA0016PO-96T 96T
EUR 618.75

Horse tumor necrosis factor α(TNF-α)ELISA Kit

YLA0018HO-48T 48T
EUR 525

Horse tumor necrosis factor α(TNF-α)ELISA Kit

YLA0018HO-96T 96T
EUR 645

Bovine Tumor necrosis factor α,TNF-α ELISA Kit

YLA0020BO-48T 48T
EUR 502.5

Bovine Tumor necrosis factor α,TNF-α ELISA Kit

YLA0020BO-96T 96T
EUR 618.75

Chicken Tumor necrosis factor α,TNF-α ELISA Kit

YLA0021CH-48T 48T
EUR 540

Chicken Tumor necrosis factor α,TNF-α ELISA Kit

YLA0021CH-96T 96T
EUR 645

Rabbit Tumor necrosis factor α,TNF-α ELISA KIT

YLA0025RB-48T 48T
EUR 465

Rabbit Tumor necrosis factor α,TNF-α ELISA KIT

YLA0025RB-96T 96T
EUR 600

Hamster Tumor necrosis factor α,TNF-α ELISA Kit

YLA0031HA-48T 48T
EUR 540

Hamster Tumor necrosis factor α,TNF-α ELISA Kit

YLA0031HA-96T 96T
EUR 645

Monkey Tumor necrosis factor α,TNF-α ELISA Kit

YLA0031MK-48T 48T
EUR 540

Monkey Tumor necrosis factor α,TNF-α ELISA Kit

YLA0031MK-96T 96T
EUR 645

Sheep Tumor necrosis factor α(TNF-α)ELISA KIT

YLA0031SH-48T 48T
EUR 540

Sheep Tumor necrosis factor α(TNF-α)ELISA KIT

YLA0031SH-96T 96T
EUR 645

Canine Tumor necrosis factor α,TNF-α ELISA Kit

YLA0032CA-48T 48T
EUR 502.5

Canine Tumor necrosis factor α,TNF-α ELISA Kit

YLA0032CA-96T 96T
EUR 618.75

GOAT Tumor necrosis factor α,TNF-α ELISA KIT

YLA0042GO-48T 48T
EUR 465

GOAT Tumor necrosis factor α,TNF-α ELISA KIT

YLA0042GO-96T 96T
EUR 600

Mouse Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0097MO-48T 48T
EUR 465

Mouse Tumor necrosis factor α(TNF-α)ELISA Kit

YLA0097MO-96T 96T
EUR 600

Cat Tumor necrosis factor α,TNF-α ELISA Kit

YLA0114CT-48T 48T
EUR 547.5

Cat Tumor necrosis factor α,TNF-α ELISA Kit

YLA0114CT-96T 96T
EUR 637.5

Rat Tumor necrosis factor α,TNF-α ELISA Kit

YLA0118RA-48T 48T
EUR 465

Rat Tumor necrosis factor α,TNF-α ELISA Kit

YLA0118RA-96T 96T
EUR 600

TNF Alpha Monoclonal Antibody

ABM40205-003ml 0.03ml
EUR 189.6
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

TNF Alpha Monoclonal Antibody

ABM40205-01ml 0.1ml
EUR 346.8
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

TNF Alpha Monoclonal Antibody

ABM40205-02ml 0.2ml
EUR 496.8
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

TNF Alpha Monoclonal Antibody

ABM40206-003ml 0.03ml
EUR 189.6
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

TNF Alpha Monoclonal Antibody

ABM40206-01ml 0.1ml
EUR 346.8
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

TNF Alpha Monoclonal Antibody

ABM40206-02ml 0.2ml
EUR 496.8
Description: A monoclonal antibody for detection of TNF Alpha from Human, Mouse, Rat. This TNF Alpha antibody is for WB, IHC-P. It is affinity-purified from mouse ascites by affinity-chromatography using epitope-specific immunogenand is unconjugated. The antibody is produced in mouse by using as an immunogen synthetic peptide

pAb rabbit anti-rat TNF-α

CT062 0.5 mg
EUR 220.8

mAb mouse anti-human TNF-α

CT258 0.5 mg
EUR 312

mAb mouse anti-human TNF-α

CT260 0.5 mg
EUR 312

mAb mouse anti-human TNF-α

CT271 0.5 mg
EUR 312

Recombinant mouse TNF-α (E.coli-derived)

CT304 10 µg
EUR 291.6

Recombinant mouse TNF-α (E.coli-derived)

CT305 100 µg
EUR 717.6

pAb rabbit anti-mouse TNF-α

CT315 0.5 mg
EUR 220.8

TNF-α (31-45), human (TFA)

HY-P1860A 5mg
EUR 1176

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

CN-00228G1 96T
EUR 564

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

CN-00228G2 48T
EUR 384

Guinea pig Tumor necrosis factor α(TNF-α)ELISA Kit

QY-E90069 96T
EUR 573.6

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

YLA0006GP-48T 48T
EUR 540

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

YLA0006GP-96T 96T
EUR 645

HSP90 α Monoclonal antibody(1F6)

44076-100ul 100ul
EUR 302.4

HSP90 α Monoclonal antibody(7D6)

44078-100ul 100ul
EUR 302.4

α-tubulin Mouse Monoclonal Antibody

37981-100ul 100ul
EUR 302.4

α-tubulin Mouse Monoclonal Antibody

37981-50ul 50ul
EUR 224.4

Genorise® Guinea Pig TNF-α polyclonal antibody

GR174002 100 µg
EUR 477.6

Genorise® Biotinylated Hamster TNF-α polyclonal antibody

GR209002 100 µg
EUR 477.6

Genorise® Biotinylated Rabbit TNF-α Polyclonal antibody

GR191018 50 mg
EUR 477.6

Genorise® Biotinylated Feline TNF-α polyclonal antibody

GR196002 100 µg
EUR 477.6

Genorise® Biotinylated Monkey TNF-α Polyclonal antibody

GR197014 100 mg
EUR 477.6

Anti-Thioredoxin / TRX (6C10)

YF-MA15994 100 ug
EUR 435.6
Description: Mouse monoclonal to Thioredoxin / TRX

Recombinant Human Tumor Necrosis Factor-α/TNF-α High Active Mutant

C036-10ug 10ug
EUR 181.2
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Tumor Necrosis Factor-α/TNF-α High Active Mutant

C036-1mg 1mg
EUR 2154
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Tumor Necrosis Factor-α/TNF-α High Active Mutant

C036-500ug 500ug
EUR 1527.6
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Recombinant Human Tumor Necrosis Factor-α/TNF-α High Active Mutant

C036-50ug 50ug
EUR 412.8
Description: Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Human IFN-γ / TNF-α FluoroSpot kit

CT851-F2 2-plate
EUR 694.8

Monkey IFN-γ / TNF-α FluoroSpot kit

CT901-F2 2-plate
EUR 694.8

Genorise® Recombinant Rat TNF-α protein

GR125003 5 µg
EUR 342

Genorise® Chicken TNF-α Expression Plasmid

GR148005 10 µg
EUR 262.8

Nori® duck TNF-α ELISA Kit

GR149025 96-well
EUR 553.2

Nori® Hamster TNF-α ELISA Kit

GR172146 96-well
EUR 553.2

Genorise® Recombinant turkey TNF-α protein

GR185003 5 mg
EUR 342

Nori® Turkey TNF-α ELISA Kit

GR187025 96-well
EUR 553.2

Nori® Feline TNF-α ELISA Kit

GR188146 96-well
EUR 553.2

Nori® Ferret TNF-α ELISA Kit

GR201055 96-well
EUR 553.2

Nori® Salmon TNF-α ELISA Kit

GR202055 96-well
EUR 553.2

Nori® Zebrafish TNF-α ELISA Kit

GR206055 96-well
EUR 553.2

Sex-based dimorphism of anticancer immune response and molecular mechanisms of immune evasion

Purpose: We previously demonstrated that sex influences response tovimmune-checkpoint inhibitors. Here we investigate sex-based differences in the molecular mechanisms of anticancer immune-response and immune evasion in patients with NSCLC.

Experimental design: We analyzed a) transcriptome-data of 2575 early-stage NSCLCs from 7 different datasets; b) 327 tumor-samples extensively characterized at the molecular level from the TRACERx lung study; c) two independent cohorts of respectively 329 and 391 patients with advanced NSCLC treated with anti-PD1/anti-PDL1 drugs.

Results: As compared with men, the tumor microenvironment (TME) of women was significantly enriched for a number of innate and adaptive immune cell-types, including specific T-cell subpopulations. NSCLCs of men and women exploited different mechanisms of immune evasion.

The TME of females was characterized by significantly greater T-cell dysfunction status, higher expression of inhibitory immune-checkpoint molecules and higher abundance of immune-suppressive cells, including Cancer Associated Fibroblasts, MDSCs and Regulatory T-cells. By contrast, the TME of males was significantly enriched for a T-cells excluded phenotype.

We reported data supporting impaired neoantigens presentation to immune system in tumors of men, as molecular mechanism explaining the findings observed. Finally, in line with our results, we showed significant sex-based differences in the association between TMB and outcome of patients with advanced NSCLC treated with anti-PD1/PDL1 drugs.

Conclusions: We demonstrated meaningful sex-based differences of anticancer immune response and immune evasion mechanisms, that may be exploited to improve immunotherapy efficacy for both women and men.

Serum high-molecular-weight adiponectin and response to dapagliflozin in patients with type 2 diabetes and non-alcoholic fatty liver disease

A better baseline renal function is associated with a better response to sodium-glucose co-transporter-2 inhibitors in patients with type 2 diabetes. Low serum adiponectin is associated with visceral fat accumulation and hepatic steatosis.

  • We investigated the relationship between baseline serum adiponectin and glycemic response to dapagliflozin in patients with type 2 diabetes and non-alcoholic fatty liver disease (NAFLD). In a randomized, active-controlled, open-label trial, 57 patients with type 2 diabetes and NAFLD were randomized to either the dapagliflozin (5 mg/d) group or the control group.
  • Both groups were treated for 24 weeks. Serum high-molecular-weight (HMW) adiponectin was measured with an ELISA kit. Visceral fat area (VFA) was measured by dual bioelectrical impedance analysis.
  • Hepatic steatosis was assessed by the controlled attenuation parameter (CAP) measured by a transient elastography (FibroScan). Treatment with dapagliflozin significantly decreased HbA1c from 8.4%±1.5% at baseline to 7.4%±1.2% at 24 weeks. Both VFA and CAP decreased in the dapagliflozin group.
  • Baseline serum HMW adiponectin was negatively correlated with changes in HbA1c from baseline to 24 weeks with dapagliflozin therapy. In the multivariate analysis, baseline HbA1c (β=-0.559, p=0.002) and serum HMW adiponectin (β=0.471, p=0.010) were independent determinants for the change (reduction) in HbA1c.
  • In the dapagliflozin group, the change in HbA1c was positively correlated with the changes of CAP, but negatively correlated with the change in serum HMW adiponectin. In conclusion, a lower serum level of HMW adiponectin was associated with a better response to dapagliflozin in patients with type 2 diabetes and NAFLD.Trial registration numberUMIN000022155.

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